In Vitro Plant Regeneration of Tomato (Lycopersiconesculentum)

The experiment was carried out at the Genetic Engineering Laboratory of the Department of Genetics and Plant Breeding, Sylhet Agricultural University, Sylhet to develop a suitable protocol for high frequency plant regeneration of tomato (Lycopersicon esculentum). An efficient system for high frequency plant regeneration system of L. esculentum was developed through investigating various factors such as combination of plant growth regulators; explant type, age and ethylene biosynthesis inhibitor AgNO3. To observe the suitable medium for callus initiation and shoot