Boiling is an Efficient Way to Purify Recombinant Allergen Lit v 1 from Whiteleg Shrimp (Litopenaeus vannamei)
Abstract
Background: The whiteleg shrimp (Litopenaeus vannamei) allergen protein Lit v 1 is tropomyosin with a double-stranded alpha-helical coiled-coil structure, which is so stable that the native structure can be easily restored even after heating. Methods: Recombinant Lit v 1 was expressed in Escherichia coli (E. coli) and E. coli were lysed by ultra sonication or boiling.
Article Information
| Journal | SF Journal of Immunology |
| Article Type | Research Article |
| Volume | 1 |
| Issue | 1 |